微生物聚焦的微流控芯片研究

Research on Microfluidic Chips for Particle Focusing

摘要:

针对流式细胞仪中聚焦细菌、酵母菌等小直径微生物困难的问题,开展微生物聚焦的微流控芯片研究。在传统直通道的基础上设计新型方形通道,建立方形通道微流控芯片的有限元模型,通过流体与运动轨迹的有限元仿真实现对通道宽度、长度等结构参数的优化设计。通道宽度为20 μm、单位长度为80 μm时,粒子仿真运动范围为通道的63%。提出了基于硅基衬底的微流控芯片制备方法,制备出方形通道微流控芯片实物。搭建了流式细胞仪原理样机,对方形通道微流控芯片进行了验证实验。实验结果表明,通道总长度约为2.16 cm,酵母菌实际运动范围为70%,表明微流控芯片可达到聚焦酵母菌的设计要求。

Abstract:

To address the challenge of focusing small-diameter microorganisms, such as bacteria and yeast, in flow cytometry, we conducted a study on microfluidic chips for microorganism focusing. A novel square channel was developed based on traditional straight-channel designs. A finite element model of the square-channel microflu idic chip was established to enable the optimization of structural parameters, including the channel width and length, through finite element simulations of fluid flow and particle trajectories. The simulation showed that when the chan nel width was 20 μm, and the unit length was 80 μm, particles were focused within 63% of the channel. A silicon based substrate was used to fabricate the square-channel microfluidic chip, and a prototype flow cytometer was con structed to test it. The experimental results indicate that with a total channel length of approximately 2.16 cm, the actual focusing range for yeast cells reached 70%, thus satisfying the design requirements for yeast focusing.